Overview

Product name
Hemin Assay Kit


Product overview
The Hemin Assay is a simple, highly sensitive assay to measure hemin levels in various biological samples. In the assay, the pseudoperoxidase activity of hemin converts a colorless, nonfluorescent probe to a strongly colored (detected by spectrophotometry at OD = 570 nm) and fluorescent (Ex 535, Em 587) compound. Therefore, hemin can be detected using both colorimetric assay (detection range is 10-250 pmol) and fluorescence assay (detection range is 1-25 pmol).


Tested applications
Functional Studiesmore details

Properties

Storage instructions
Store at -20°C, Stable for 6 months at -20°C



    

        

            
Components
            
Identifier
            
100 tests
        
    
    

        

            
DMSO (anhydrous)
            
Brown
            
1 x 400µl
        
        

            
Enzyme Mix
            
Blue
            
1 vial
        
        

            
Hemin Assay Buffer
            
WM
            
1 x 25ml
        
        

            
Hemin Assay Kit
            
ab65332
            
100 tests
        
        

            
Hemin Probe
            
Red
            
1 vial
        
        

            
Hemin Standard (1 nM) (Lyophilised)
            
Yellow
            
1 vial
        
        

            
Hemin Substrate
            
Green
            
1 x 1ml
        
    


http://www.abcam.com/Hemin-Assay-Kit-ab65332.html